shan@uncfsu.edu

 Research Interests

• Atomic Force Microscopy of Surface Interaction of Biological Species (DNA, proteins and drugs)

• Fabrication and Characterization of Nanomaterials (Nanoarray, Nanotube, Nanoparticles) and Bio-functionalized Nanomaterials

• Electrochemical and Optical Biosensor Development 

A Polyaniline  honeycomb film generated by electro- polymerization of aniline in the interstitial voids of PE2-PS Particle assembly

Teaching

• Instrumental Analysis, CHEM 310

• Analytical Chemistry, CHEM 210

• General Chemistry I, CHEM 140

• General Chemistry II, CHEM 160

• Physical Sciences, NSCI 110

Baicalein  effectively dis- aggregates a-synuclein fibrils of  (Observed by in-situ MAC-AFM in solution) (Click the image to see the movie)

1. Hong, D.; Han, S.; Fink, A. L.,  N. Uversky, N. V. Characterization of the Non-Fibrillar α-Synuclein Oligomers(2011), Protein and Peptide Letters,  18( 3), pp. 230-240.

2. Qin, Z.; Hu, D.; Han, S.; Hong, D.; Fink, A.L. Role of different regions of a -synuclein in the assembly of fibrils. Biochemistry (2007), 46(46), 13322-13330.

3. Qin, Z.; Hu, D.; Han, S.; Reaney, S. H.; Di Monte, D. A.; Fink, A.L. Effect of 4-hydroxy-2-nonenal modification on a -synuclein aggregation. Journal of Biological Chemistry (2007), 282(8), 5862-5870.

4. Martinez, Z.; Zhu, M; Han, S.; Fink, A.L. GM1 specifically interacts with a-synuclein and inhibits fibrillation. Biochemistry (2007), 46(7), 1868-1877.

5. Wang, J.;  Zhu X.; Han, S. Electrochemistry of bilayer lipid membranes. Recent Res. Devel. Biophys., 5(2006): 319-332.

6. Wang, Y., Han, S., Briseno, A.L., Sanedrin, R.J.G., and Zhou, F., A modified nanosphere lithography for the fabrication of aminosilane/polystyrene nanoring arrays and the subsequent attachment of gold or DNA-capped gold nanoparticles. Journal of Materials Chemistry, 2004, 14. 3488-3494.

7.  Zhu, M., Han, S., Zhou, F., Carter, S.A., and Fink, A.L., Annular oligomeric Amyloid Intermediates Observed by in Situ Atomic Force Microscopy. Journal of Biological Chemistry, 2004. 279(23):  244529.

8. Zhu, M., Rajamani, S., Kaylor, J., Han, S., Zhou, F., and Fink, A.L., The flavonoid baicalein inhibits fibrillation of alpha-synuclein and disaggregates existing fibrils. Journal of Biological Chemistry, 2004. 279(26): 26846-57.

9. Briseno, A.L., Han, S., Rauda, I.E., Zhou, F., Toh, C.-S., Nemanick, E.J., and Lewis, N.S., Electrochemical Polymerization of Aniline Monomers Infiltrated into Well-Ordered Truncated Eggshell Structures of Polyelectrolyte Multilayers. Langmuir, 2004. 20(1):  219 -226.

10. Han, S., Sun, X.Z., Wang, J., Li, X., Zhou, F.M., and Momand, J., AFM studies of interactions of proteins with surface-confined ds DNA. Biophysical Journal, 2003. 84(2):  471A-471A.

11. Han, S., Ralin, D., Wang, J., Li, X., and Zhou, F., Attachment of Monoclonal Antibody Molecules to Surface-Confined DNA Duplexes Imaged by Atomic Force Microscopy. Langmuir, 2003. 19(21): 8943-89509.

12. Chickneyan, Z.S., Briseno, A.L., Shi, X., Han, S., Huang, J., and Zhou, F., Polyelectrolyte-Mediated Assembly of Copper Phthalocyanine Tetrasulfonate Multilayers and the Subsequent Production of Nanoparticulate Copper Oxide Thin Films. Journal of Nanoscience and Nanotechnology, 2004. 4:  628-634.

13. Sun, X.Z., Vinci, C., Makmura, L., Han, S., Tran, D., Nguyen, J., Hamann, M., Grazziani, S., Sheppard, S., Gutova, M., Zhou, F., Thomas, J., and Momand, J., Formation of Disulfide Bond in p53 Correlates with Inhibition of DNA Binding and Tetramerization. Antioxidants & Redox Signaling, 2003. 5(5):  655-665.

14. Han, S., Briseno, A.L., Shi, X., Mah, D.A., and Zhou, F., Polyelectrolyte-coated nanosphere lithographic patterning of surfaces: Fabrication and characterization of electropolymerized thin polyaniline honeycomb films. Journal of Physical Chemistry B, 2002. 106(25): 6465-6472.

15. Han, S., Shi, X., and Zhou, F., Polyelectrolyte Hollow Sphere Lithographic Patterning of Surfaces: Construction of 2-Dimensional Well-Ordered Metal Arrays. Nano Letters, 2002. 2(2): 97-100.

16. Shi, X., Han, S., Sanedrin, R.J., Galvez, C., Ho, D.G., Hernandez, B., Zhou, F., and Selke, M., Formation of Cobalt Oxide Nanotubes: Effect of Intermolecular Hydrogen Bonding between Co(III) Complex Precursors Incorporated onto Colloidal Templates. Nano Letters, 2002. 2(4):  289-293.

17. Shi, X., Han, S., Sanedrin, R.J., Zhou, F., and Selke, M., Synthesis of Cobalt Oxide Nanotubes from Colloidal Particles Modified with a Co(III)-Cysteinato Precursor. Chemistry of Materials, 2002. 14(4): 1897-1902.

18. Huang, E., Satjapipat, M., Han, S., and Zhou, F., Surface Structure and Coverage of an Oligonucleotide Probe Tethered onto a Gold Substrate and Its Hybridization Efficiency for a Polynucleotide Target. Langmuir, 2001. 17(4):  1215-1224.

19. 14. Han, S., Lin, J., Satjapipat, M., Baca, A.J., and Zhou, F., A three-dimensional heterogeneous DNA sensing surface formed by attaching oligodeoxynucleotide-capped gold nanoparticles onto a gold-coated quartz crystal. Chemical Communications (Cambridge, United Kingdom), 2001(7):  609-610.

20. Han, S., Lin, J., Zhou, F., and Vellanoweth, R.L., Oligonucleotide-Capped Gold Nanoparticles for Improved Atomic Force Microscopic Imaging and Enhanced Selectivity in Polynucleotide Detection. Biochemical and Biophysical Research Communications, 2000. 279:  265-269.

21. Han, S., Zhu, M., Yuan, Z., and Li, X., A methylene blue-mediated enzyme electrode for the determination of trace mercury(II), mercury(I), methylmercury, and mercury-glutathione complex. Biosensors & Bioelectronics, 2001. 16(1-2):  9-16.

22. Zhu, M., Han, S., and Yuan, Z., beta-cyclodextrin polymer as the immobilization matrix for peroxidase and mediator in the fabrication of a sensor for hydrogen peroxide. Journal of Electroanalytical Chemistry, 2000. 480(1-2):  255-261.

23. Han, S., Li, X., Guo, G., Sun, Y., and Yuan, Z., Voltammetric measurement of microorganism populations. Analytica Chimica Acta, 2000. 405(1-2): 115-121.

24. Yuan, Z., Zhu, M., and Han, S., Supramolecular inclusion complex formation and application of b-cyclodextrin with heteroanthracene ring cationic dyes. Analytica Chimica Acta, 1999. 389(1-3):  291-298.

Patent

1. Rassman, W., Ralin, D., Zhou, F., and Han, S., Method and apparatus for recognizing molecular compounds, in U.S. Pat. Appl. Publ. 2003, (USA). Us. p. 17 pp.

Recent Presentations

1. Guadamuz, Dalia; Tinoco, Virna O.; Lee, Andrew D.; Juma, Booker; Han, Shubo. Cellulose nanocomposites in grape seed extracts help antioxidants in preventing alpha synuclein aggregation. Abstracts of Papers, 235th ACS National Meeting, New Orleans, LA, United States, April 6-10, 2008 .
2. Hodges, N. Ashley; Tonjanika, Boyd; Han, Shubo. The antioxidants in natural plants affecting formation of alpha synuclein nanobioparticles. Abstracts of Papers, 234th ACS National Meeting, Boston, MA, United States, August 19-23, 2007 (2007), AGRO-060.

3. N. Ashley Hodges, Tonjanika Boyd, Lorra S. Moses, and Shubo Han.Voltammetric study of methionine oxidation in alpha synuclein oligomerization.Abstracts of Papers, The 233rd ACS National Meeting, Chicago, IL, March 25-29, 2007, CHED-381.

4. Ward, Saquanta Y.; Han, Shubo. Surface interaction a -synuclein oligomers with phosphatidylcholine lipid. Abstracts of Papers, 231st ACS National Meeting, Atlanta, GA, United States, March 26-30, 2006 (2006), CHED-791.

5. Rauda, Iris E.; Han, Shubo; Zhou, Feimeng. Studies of amyloidogenic protein interactions at the surface/solution interface via atomic force microscopy. Abstracts of Papers, 229th ACS National Meeting, San Diego, CA, United States, March 13-17, 2005.

6. Wang, Yue; Han, Shubo; Zhou, Feimeng; Sogamonyan, Tagohi; Momond, Jamail. Characterization of Localized Interactions of p53 with Oligonucleotides Representing MDM Promoter Immobilized onto Silicon Surface. Abstracts, 39th Western Regional Meeting of the American Chemical Society, Sacramento, CA, United States, October 27-30 (2004).

Links