Research Expertise and Interests

 

 

Research Expertise:

 

1. DNA markers: RFLPs, Microsarellites or SSRs, AFLPs, and RAPDs, etc.

2. Genetic mapping of QTL for important agronomic traits in Soybean:

                 - Soybean cyst nematode (SCN) resistance.

                 - Sudden death syndrome (SDS) resistance.

                 - Manganese (Mn) toxicity resistance.

                 - Seed isoflavone content.

                 - Seed yield, plant height, lodging, etc.

                 - Root and shoot traits, etc.

3. Construction of genetic linkage maps of important crops such as soybean.

4. Construction of BAC libraries for important crops (soybean, chickpea, etc.).

5. Regeneration in vitro of herbaceous and woody plant species.

6. Plant secondary metabolism (soybean isoflavones, morphinan alkaloids, etc.).

 

Research Projects:

 

 Our research projects and interests here at Fayetteville State University are as follow:

1. Increase the number of SSR markers on the soybean genome.

2. Genetic mapping of QTL for important agronomic traits in Soybean:

     - Soybean cyst nematode (SCN) resistance.

     - Sudden death syndrome (SDS) resistance.

     - Manganese (Mn) toxicity resistance.

     - Isoflavones, saponins, anthocyanins, and other metabolites contents.

     - Seed yield, seed weight, and 100 seed weight.

     - Time to flowering, plant height, lodging, etc.

3. Construction of genetic linkage maps of soybean and other plants.

4. Construction of BAC libraries for soybean, chickpea, etc.

5. Regeneration in vitro of herbaceous and woody plant species.

6. Plant secondary metabolism (soybean isoflavones, morphinan alkaloids, etc.).

 

 We collaborate extensively with Drs. K Meksem and DA Lightfoot, Southern Illinois University, Carbondale, IL.

 

Research Opportunities for Students:

I am continuously looking for motivated undergraduate and graduate (Master's level) students who are interested in working on plant genetics, genomics, and biotechnology research projects.

Students can register in BIOL 630 Topics in Biology, BIOL  Independent Research, or BIOL 690 Research and receive credit.

If you are interested in plant genetics, genomics, and biotechnology research, please contact me at 910-672-1114; send me an email at mkassem@uncfsu.edu; or stop by LS 122 to discuss these research opportunities.

 

 

 

Publications:  

 

A. Journal Articles:

 

Karangula UB, MA Kassem, L  Gupta, and DA Lightfoot. Genome Architecture Underlies Seed Yield In Soybeans Resistant to Heterodera glycines. Crop Science 2008. In review.

 

Jacobson J, A Alcivar, J Rainho, J Mokzerniuk, K Shah, J Arturo, DA Lightfoot, and MA Kassem. Genomic Regions Containing QTL for Plant Height, Internodes Length, and Flower Color in Soybean [Glycine max (L.) Merr.]. BIOS A Quarterly Journal of Biology 78 (4): xx-xx, January 2008. In press.

 

Kassem MA, K Meksem, AJ Wood, and DA Lightfoot. A Microsatellite Map Developed from Late Maturity Germplasm ‘Essex’ by ‘Forrest’ Detects Four QTL for Soybean Seed Yield Expected from Early Maturing Germplasm. Reviews in Biology & Biotechnology 6 (1): 2-10, January 2007.

Kassem MA, K Meksem, AJ Wood, and DA Lightfoot. Additional QTL for SDS and SCN resistances in a soybean recombinant inbred line population of Essex x Forrest. Reviews in Biology & Biotechnology 6 (1): 11-19, January 2007. 

 

Alcivar A, J Jacobson, J Rainho, K Meksem, DA Lightfoot, and MA Kassem. Genetic Analysis of Soybean Plant Height, Hypocotyl and Internode Lengths. Journal of Agricultural, Food, and Environmental Sciences 1 (1): March 2007.

 

Kassem MA, J Shultz, K Meksem, Y Cho, AJ Wood, MJ Iqbal, and DA Lightfoot. An Updated ‘Essex’ by ‘Forrest’ Linkage Map and First Composite Map of QTL Underlying Six Soybean Traits. Theor Appl Genet 2006, 113 (6): 1015-1026.

 

Shultz JL, D Kurunam, K Shopinski, MJ Iqbal, S Kazi, K Zobrist, R Bashir, S Yaegashi, N Lavu, AJ Afzal, CR Yesudas, MA Kassem, C Wu, HB Zhang, CD Town, K Meksem, and DA Lightfoot. The Soybean Genome Database (SoyGD): a browser for display of duplicated, polyploid, regions and sequence tagged sites on the integrated physical and genetic maps of Glycine max. Nucleic Acids Research, 2006 (34): D758-D765.

Lightfoot DA, VN Njiti, PT Gibson, MA Kassem, JM Iqbal, and K Meksem. Registration of the Essex by Forrest Recombinant Inbred Line (RIL) Mapping Population. Crop Science 45(4): 1678-1781, July 2005.

Kassem MA, K Meksem, CH Kang, VN Njiti, VY Kilo, AJ Wood, and DA Lightfoot. Loci Underlying Resistance to Manganese Toxicity Mapped in a Soybean Recombinant Inbred Line population of 'Essex' x 'Forrest'. Plant and Soil. 260: 197-200, 2004a.

Kassem MA, Meksem K, Iqbal MJ, Njiti VN, Banz WJ, Winters TA, Wood A, Lightfoot DA. Definition of Soybean Genomic Regions That Control Seed Phytoestrogen Amounts. J. Biomed & Biotech 1(1): 52-60, 2004b.

Njiti VN, Meksem K, Iqbal MJ, JE Johnson, Kassem MA, KF Zobrist, VY Kilo, D A Lightfoot. Common Loci Underlie Field Resistance to Soybean Sudden Death Syndrome in Forrest, Pyramid, Essex, and Douglas. Theor Appl Genet 104(2/3): 294-300, 2002.

Yuan J, VN Njiti, K Meksem, MJ Iqbal, K Triwitayakorn, MA Kassem, GT Davis, ME Schmidt, DA Lightfoot. Quantitative trait loci in Two Soybean Recombinant Inbred Line Populations Segregating for Yield and Disease Resistance. Crop Science 42: 271-277, 2002.

Cho Y, Njiti VN, Chen X, Triwatayakorn K, Kassem MA, Meksem K, Lightfoot DA, Wood AJ. Quantitative Trait Loci Associated with Foliar Trigonelline Accumulation in Glycine Max L. J. Biomed & Biotech 2(3): 151-157, 2002.

Iqbal MJ, K Meksem, VN Njiti, MA Kassem, and DA Lightfoot. Microsatellite markers identify three additional quantitative trait loci for resistance to soybean sudden death syndrome (SDS) in Essex x Forrest RILs. Theor Appl Genet 102(2/3): 187-192, 2001.

Men AM, K Meksem, MA Kassem, D Lohar, J Stiller, DA Lightfoot, PM Gresshoff. A Bacterial Artificial Chromosome of Lotus Japonicus Constructed in an Agrobacterium tumefaciens - Transformable Vector. Molecular Plant-Microbe Interactions 14(3): 422-425, 2001.

Kassem MA and Jacquin A. Somatic Embryogenesis, Rhizogenesis, and Morphinan Alkaloids Production in Two Species of Opium Poppy. J. Biomed. & Biotech. 1(2): 70-78, 2001.

Meksem K, VN Njiti, WJ Banz, MJ Iqbal, MA Kassem, DL Hyten, J Yuang, TA Winters, and DA Lightfoot. Genomic regions that underlie soybean seed isoflavone content. J. Biomed. & Biotech. 1(1): 38-44, 2001.
 

 

 

B. Selected Presentations in National & International Conferences:

 

 

Research Resources and Links: